![]() Iberian pig mesenchymal stem/stromal cells from dermal skin, abdominal and subcutaneous adipose tissues, and peripheral blood: in vitro characterization and migratory properties in inflammation, Stem Cell Research & Therapy, Volume 9 (2018) no. Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT), Cytotherapy, Volume 15 (2013) no. It suggests that MSCs may play a significant role in adipose tissue and skeletal muscle homeostasis and may influence later growth and body composition in growing animals. This study shows that the proportions of some MSC populations were affected by hygiene of housing conditions in a tissue-dependent manner in pigs of both RFI lines. The proportion of CD45 -CD56 -CD34 - cells increased (P < 0.05) whereas the proportion of CD45 -CD56 +CD34 - tended to decrease (P < 0.1) in pigs housed in poor conditions. ![]() In adipose tissue, the two abundant MSC populations were CD45 -CD56 -CD34 - and CD45 -CD56 +CD34. For the CD45 -CD56 -CD34 - cell population, present in low proportion, there was an interaction between hygiene condition and genetic line (P < 0.05) with a decrease in low RFI pigs housed in poor hygiene conditions. Of these two cell populations, only the proportions of CD45 -CD56 +CD34 +CD140a + cells increased (P ≤ 0.05) in pigs housed in poor hygiene as compared with pigs in good hygiene conditions. In muscle, the CD45 -CD56 +CD34 -CD140a + and CD45 -CD56 +CD34 +CD140a + cell populations were abundant. Adipose tissue and muscle shared some common MSC populations although MSC diversity was higher in muscle than in adipose tissue. Adipose and muscle cell populations from the SVF were phenotyped by flow cytometry using antibodies that targeted different cell surface markers (CD45 to separate hematopoietic cells from MSCs CD34, CD38, CD56 and CD140a to identify MSC populations with adipogenic and/or myogenic potential). Samples of subcutaneous adipose tissue and longissimus skeletal muscle were collected, and cells from the stromal vascular fraction (SVF), which includes mesenchymal stromal/stem cell populations, were isolated from each tissue. After six weeks, pigs were slaughtered (n = 30 5-9/group). ![]() At the age of 12 weeks, Large White pigs from two lines divergently selected for low and high RFI were housed in two contrasting hygiene conditions (good vs poor). We hypothesized that the impact of hygiene of housing conditions during growth on the proportions of MSCs in adipose tissue and skeletal muscle may differ between pigs with different residual feed intake (RFI), a measure of feed efficiency. During growth, the increase in the number of cells results from the recruitment of different populations of multipotent mesenchymal stromal/stem cells (MSCs) residing in the tissues. In pigs, the ratio between lean mass and fat mass in the carcass determines production efficiency and is strongly influenced by the number and size of cells in tissues.
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